Application
Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody at a dilution of 1:10000.| Product Name | Histone H3T3ph (phospho Thr3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (phospho Thr3) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3 (phospho Thr3). The exact sequence is proprietary. |
| Modification | p-Thr3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H3 phospho histone cluster 1 H3a Histone H3 (phospho Thr3) phospho Histone H3 (T3) HIST1H3A phospho Histone H3 H3FA Histone H3 phospho Thr3 Histone H3 phospho T3 phospho Histone H3 (pT3) phospho Histone H3 (pThr3) |
|---|---|
| Target | Histone H3 (phospho Thr3) |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3 (phospho Thr3) antibody detects Histone H3 (phospho Thr3) protein by western blot analysis. Un-treated (-) and treated (+, 0.1uM Calyculin for 30 minutes + phosphatase inhibitor) HeLa whole cell extracts (2 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (phospho Thr3) antibody at a dilution of 1:10000.| Positive Control | HeLa (untreated) , HeLa (0.1uM Calyculin treatment for 30 minutes+ phosphatase inhibitor) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
