Application
Western Blot of recombinant Histone H2A, H2B,H3.1, H3.3 and H4 proteins, using RM212 at0.2 ug/mL. RM212 is specific to Histone H4.Application
Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM212 at 0.2 ug/mL, showed endogenous Histone H4 in A375, HEK293, HeLa and SK-MEL-2 cells.| Product Name | Histone H4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM212] to Histone H4Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the C-terminus of human Histone H4. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM212 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIST4H4 H4/p histone cluster 4 H4 Histone H4 antibody |
|---|---|
| Target | Histone H4 |
| Tissue Specificity | This antibody reacts to the Histone H4 protein, independent of post-translational modifications. No cross reactivity with other histone proteins. |
Application
Western Blot of recombinant Histone H2A, H2B,H3.1, H3.3 and H4 proteins, using RM212 at0.2 ug/mL. RM212 is specific to Histone H4.
Application
Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using RM212 at 0.2 ug/mL, showed endogenous Histone H4 in A375, HEK293, HeLa and SK-MEL-2 cells.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
