Application
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 ug/mL,showed a band of Histone monomethylated at Arginine3 in HeLa cells.Application
RM195 specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylatedArg 3 (R3me2a) or symmetric dimethylated Arg 3(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.| Product Name | Histone H4R3me1 (Monomethyl Arg3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM195] to Histone H4 (Monomethyl Arg3)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008] |
| Immunogen | A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3). |
| Modification | Monomethyl Arg3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM195 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIST4H4 H4/p histone cluster 4 H4 H4R3Me1 antibody |
|---|---|
| Target | Histone H4 (Monomethyl Arg3) |
| Tissue Specificity | This antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4. |
Application
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM195 at 0.5 ug/mL,showed a band of Histone monomethylated at Arginine3 in HeLa cells.
Application
RM195 specifically reacts to Histone H4 mono-methylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylatedArg 3 (R3me2a) or symmetric dimethylated Arg 3(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
