Application
Sample(30 ug whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:1000| Product Name | HLA DR beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HLA-DRB4 (major histocompatibility complex, class II, DR beta 4)HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA DR beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DR-13 antibody DR-14 antibody DR13 antibody DR14 antibody DRB4 antibody HLA-DR4B antibody HLA-DRB4 antibody leukocyte antigen antibody human leucocyte antigen DRB4 antibody MHC HLA DR-beta chain antibody "major histocompatibility complex class I |
|---|---|
| Target | HLA-DRB4 |
| Molecular Weight(MW) | 30 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample(30 ug whole cell lysate) A:Raji 12% SDS PAGE diluted at 1:1000| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
