Application
HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments. IP samples: A549 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HLA-A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-A (major histocompatibility complex, class I, A)HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ26655 antibody HLAA antibody HLA-A antibody antigen presenting molecule antibody MHC class I antigen HLA-A heavy chain antibody leukocyte antigen class I-A antibody "HLA class I histocompatibility antigen A-1 alpha chain antibody" "major histoco |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (3) |
| Cellular Localization | Membrane |
Application
HLA-A antibody [N1C2] immunoprecipitates HLA-A protein in IP experiments. IP samples: A549 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 μg HLA-A antibody [N1C2] 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Sample (30 µg of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
