Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HLA-DMA antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE diluted at 1:1000Application
HLA-DMA antibody detects HLA-DMA protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 12 % SDS-PAGE HLA-DMA antibody dilution: 1:500| Product Name | HLA-DMA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-DMA (major histocompatibility complex, class II, DM alpha)HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-DMA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D6S222E antibody DMA antibody HLADM antibody RING6 antibody HLA-DMA antibody MHC class II antigen DMA antibody "class II histocompatibility antigen M alpha chain antibody" really interesting new gene 6 protein antibody "HLA class II histocompatibi |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HLA-DMA antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE diluted at 1:1000
Application
HLA-DMA antibody detects HLA-DMA protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 12 % SDS-PAGE HLA-DMA antibody dilution: 1:500| Positive Control | HeLa , HepG2 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
