Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HLA-DMB antibody at 1:250 dilution.Application
Western Blot analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB polyclonal antibody. A:Non-transfected lysate. B:HLA-DMB transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | HLA-DMB antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-DMB (major histocompatibility complex, class II, DM beta)HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HLA-DMB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D6S221E antibody RING7 antibody HLA-DMB antibody really interesting new gene 7 protein antibody MHC class II HLA-DMB antibody "HLA class II histocompatibility antigen DM beta chain antibody" "class II histocompatibility antigen M beta chain antibod |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Late endosome membrane , Lysosome membrane |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HLA-DMB antibody at 1:250 dilution.
Application
Western Blot analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB polyclonal antibody. A:Non-transfected lysate. B:HLA-DMB transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
