Application
Immunofluorescence analysis of methanol-fixed HeLa, using HLA-DPA1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HLA-DPA1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000| Product Name | HLA-DPA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-DPA1 (major histocompatibility complex, class II, DP alpha 1)HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-DPA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HLA-DP1A antibody HLADP antibody HLASB antibody PLT1 antibody HLA-DPA1 antibody "HLA class II histocompatibility antigen DP alpha chain antibody" MHC class II antigen antibody MHC class II DP3-alpha antibody "HLA class II histocompatibility antige |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using HLA-DPA1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HLA-DPA1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
