Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HLA-DQB2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: K562 12% SDS PAGE diluted at 1:1000| Product Name | HLA-DQB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-DQB2 (major histocompatibility complex, class II, DQ beta 2)HLA-DQB2 belongs to the family of HLA class II beta chain paralogs. Class II molecules are heterodimers consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). Polymorphisms in the alpha and beta chains specify the peptide binding specificity, and typing for these polymorphisms is routinely done for bone marrow transplantation. However this gene, HLA-DQB2, is not routinely typed, as it is not thought to have an effect on transplantation. There is conflicting evidence in the literature and public sequence databases for the protein-coding capacity of HLA-DQB2. Because there is evidence of transcription and an intact ORF, HLA-DQB2 is represented in Entrez Gene and in RefSeq as a protein-coding locus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-DQB2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HLA-DQB1 antibody HLA-DXB antibody HLA-DQB2 antibody "HLA class II histocompatibility antigen DX beta chain antibody" "DV19.1 (major histocompatibility complex class II DQ beta 2 (HLA-DXB)) antibody" MHC class II antigen DQB2 antibody "major histo |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HLA-DQB2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: K562 12% SDS PAGE diluted at 1:1000| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
