Application
HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis. Sample: Raji cells were fixed in 100% Methenol for 5 min. Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
HLA-DRA antibody detects HLA-DRA protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRA antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HLA-DRA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HLA-DRA (major histocompatibility complex, class II, DR alpha)HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-DRA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Bovine, DogBovine (82%), Dog (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ51114 antibody HLA-DRA1 antibody MLRW antibody HLA-DRA antibody MHC class II antigen DRA antibody MHC cell surface glycoprotein antibody histocompatibility antigen HLA-DR alpha antibody "HLA class II histocompatibility antigen DR alpha chain ant |
|---|---|
| Target | HLA-DRA |
| Molecular Weight(MW) | 29 kDa (note) (2) |
| Cellular Localization | Membrane |
Application
HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis. Sample: Raji cells were fixed in 100% Methenol for 5 min. Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
HLA-DRA antibody detects HLA-DRA protein by western blot analysis. Whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DRA antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
