Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HLA-E antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 D: HL-60 10% SDS PAGE diluted at 1:10000| Product Name | HLA-E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-E (major histocompatibility complex, class I, E)HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Full length human HLA-E Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686P19218 antibody EA1.2 antibody EA2.1 antibody HLA-6.2 antibody MHC antibody QA1 antibody HLA-E antibody MHC HLA-E alpha-2.1 antibody lymphocyte antigen antibody "HLA class I histocompatibility antigen alpha chain E antibody" "HLA class I |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HLA-E antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 D: HL-60 10% SDS PAGE diluted at 1:10000| Positive Control | Raji , K562 , THP-1 , HL-60 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
