Application
HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term) . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). HMGA1 immunoreactivity is localized to nucleus significantly.Application
HMGA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).| Product Name | HMGA1 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HMGA1 (C-term)This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 71~101 amino acids from the C-terminal region of human HMGA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | High mob HMGA1 High mobility group AThook protein 1 High mobility group protein A1 High mobility group protein R High mobility group AT-hook protein 1 High mobility group protein A1 High mobility group protein HMGI/HMGY HMGI(Y) HMGIY HMGA1 High |
|---|---|
| Target | HMGA1 |
| Molecular Weight(MW) | 11.676 kDa (note) |
Application
HMGA1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term) . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). HMGA1 immunoreactivity is localized to nucleus significantly.
Application
HMGA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
