Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody diluted at 1:2000.Application
HMGB2 antibody detects HMGB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HMGB2 antibody dilution: 1:500.Application
HMGB2 antibody detects HMGB2 protein by western blot analysis. Non-transfected (-), HMGB1-transfected (+) and HMGB2-transfected (+) whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody diluted at 1:3000.Application
HMGB2 antibody detects HMGB2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB2 antibody dilution: 1:1000| Product Name | HMGB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGB2 (high mobility group box 2)This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human HMGB2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (100%), Rat (100%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | high mobility group box 2 HMG2 |
|---|---|
| Target | HMGB2 |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody diluted at 1:2000.
Application
HMGB2 antibody detects HMGB2 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HMGB2 antibody dilution: 1:500.
Application
HMGB2 antibody detects HMGB2 protein by western blot analysis. Non-transfected (-), HMGB1-transfected (+) and HMGB2-transfected (+) whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB2 antibody diluted at 1:3000.
Application
HMGB2 antibody detects HMGB2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB2 antibody dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , human HMGB1-transfected 293T , human HMGB2-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
