Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HMGCL antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE HL antibody diluted at 1:1000| Product Name | HMGCL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGCL (3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase)The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMGCL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (85%), Rat (86%), Chicken (80%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HL antibody HMGCL antibody mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase antibody HMG-CoA lyase antibody 3-hydroxy-3-methylglutaryl-CoA lyase antibody "hydroxymethylglutaryl-CoA lyase mitochondrial antibody" hydroxymethylglutaricaciduria antibo |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HMGCL antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE HL antibody diluted at 1:1000| Positive Control | A549 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
