Application
WB analysis of extracts from various cell lines using HMGCR antibody.Application
IHC-P analysis of human kidney tissue using HMGCR antibody at a dilution of 1:200.Application
ICC/IF analysis of A549 cells using HMGCR antibody (green) and DAPI (blue).| Product Name | HMGCR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGCRHMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Immunogen | Recombinant protein of human HMGCR |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:200. IHC: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LDLCQ3 3-hydroxy-3-methylglutaryl-CoA reductase |
|---|---|
| Target | HMGCR |
| Molecular Weight(MW) | 95 kDa (note) |
Application
WB analysis of extracts from various cell lines using HMGCR antibody.
Application
IHC-P analysis of human kidney tissue using HMGCR antibody at a dilution of 1:200.
Application
ICC/IF analysis of A549 cells using HMGCR antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:200. IHC: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
