Application
HMGCS1 antibody detects HMGCS1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HMGCS1 antibody dilution: 1:500.Application
HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HMGCS1 antibody dilution: 1:500.Application
HMGCS1 antibody detects HMGCS1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 protein stained by HMGCS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | HMGCS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGCS1 (hydroxymethylglutaryl-CoA synthase, cytoplasmic) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMGCS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Rhesus Monkey, BovineRat (97%), Pig (95%), Chicken (82%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HMGCS antibody MGC90332 antibody HMGCS1 antibody 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody HMG-CoA synthase antibody 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase antibody "hydroxymethylglutaryl-CoA synthase cytoplasmic anti |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (8) |
| Cellular Localization | Cytoplasm |
Application
HMGCS1 antibody detects HMGCS1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HMGCS1 antibody dilution: 1:500.
Application
HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HMGCS1 antibody dilution: 1:500.
Application
HMGCS1 antibody detects HMGCS1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 protein stained by HMGCS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , HeLa , HepG2 , Mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
