Application
Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of HMGCS1 antibody (Center) in HL60 cell line lysates (35ug/lane). HMGCS1 (arrow) was detected using the purified Pab.Application
Western blot analysis of HMGCS1 antibody (Center) in mouse liver tissue lysates (35ug/lane). HMGCS1 (arrow) was detected using the purified Pab.| Product Name | HMGCS1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HMGCS1 (Internal)This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. |
| Immunogen | KLH conjugated synthetic peptide between 296~326 amino acids from the Center region of human HMGCS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | HMGCoA synthase Hydroxymethylglutaryl-CoA synthase cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase HMG-CoA synthase HydroxymethylglutarylCoA synthase cytoplasmic HMGCS 3hydroxy3methylglutaryl coenzyme A synthase HMGCS1 HMG CoA synthase |
|---|---|
| Target | HMGCS1 |
| Molecular Weight(MW) | 57.294 kDa (note) |
Application
Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of HMGCS1 antibody (Center) in HL60 cell line lysates (35ug/lane). HMGCS1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of HMGCS1 antibody (Center) in mouse liver tissue lysates (35ug/lane). HMGCS1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
