Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE HNF1 alpha antibody [N1N3] dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha protein stained by HNF1 alpha antibody [N1N3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 μg of HNF1 alpha antibody [N1N3] . Western blot analysis was performed using HNF1 alpha antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha antibody at 1:100 dilution.| Product Name | HNF1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HNF-1 alpha (HNF1 homeobox A)The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (85%), Pig (99%), Chicken (95%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HNF-1A antibody HNF1 antibody IDDM20 antibody LFB1 antibody MODY3 antibody TCF-1 antibody TCF1 antibody HNF1A antibody albumin proximal factor antibody HNF-1-alpha antibody "transcription factor 1 hepatic antibody" hepatocyte nuclear factor 1-a |
|---|---|
| Target | HNF-1 alpha |
| Molecular Weight(MW) | 67 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 7.5% SDS-PAGE HNF1 alpha antibody [N1N3] dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha protein stained by HNF1 alpha antibody [N1N3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 μg of HNF1 alpha antibody [N1N3] . Western blot analysis was performed using HNF1 alpha antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha antibody at 1:100 dilution.| Positive Control | HepG2 , Mouse liver , Rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
