Rabbit Polyclonal antibody to HNF4 alpha (Phospho Ser304)The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes,including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.
Immunogen
Peptide sequence around phosphorylation site of serine 304 (L-R-S(p)-Q-V)derived from Human HNF4 alpha .
Modification
p-Ser304
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Western blot analysis of extracts from Hela cells untreated or treated with Anisomyclin using HNF4 alpha (Phospho-Ser304) Antibody .
Application Notes
Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.