Application
Western blot analysis of HNF-4 with mouse small intestine tissue lysate| Product Name | HNF4 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HNF4 alphaHNF-4 (Hepatocyte nuclear factor 4), a member of the steroid/thyroid nuclear receptor superfamily, is a transcriptional factor which expresses in the liver, intestine, kidney, and pancreatic cells. It contains several functional domains: a ligand-independent activation domain (AF1), a zinc finger DNA binding domain, and a ligand-dependent activation domain (AF2). HNF-4 binds to a specific DNA element as a homodimer and regulates the expression of many genes, involved in glucose, fatty acid, and cholesterol metabolisms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls.: |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TCF HNF4 TCF14 FLJ39654 HNF 4 HNF4A MODY 1 MODY MODY1 |
|---|---|
| Target | HNF-4 |
| Tissue Specificity | This antibody is specific for HNF-4. |
Application
Western blot analysis of HNF-4 with mouse small intestine tissue lysate| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
