Rabbit polyclonal antibody to HNMTIn mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFIHC-PWB
IF : 1:10~50,WB
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Gene Synonyms
HNMT-S2 HNMT-S1 HMT histamine N-methyltransferase
Target
HNMT
Application
Application
Confocal IF analysis of HeLa cells using HNMT antibody (green) and DAPI (blue).
Application
IHC-P analysis of human hepatocarcinoma tissue using HNMT antibody.
Application
WB analysis of HeLa cell lysate (35ug) using HNMT antibody.
Application Notes
IF : 1:10~50,WB
Additional Information
Form
Liquid
Storage Instructions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.