Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1 antibody at 1:200 dilution.Application
Sample(30 µg of whole cell lysate) A:H1299 B:HeLa S3 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
hnRNP A1 antibody detects HNRNPA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE hnRNP A1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. hnRNP A1 antibody diluted at 1:500.Application
hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody diluted at 1:500.| Product Name | hnRNP A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hnRNP A1 (heterogeneous nuclear ribonucleoprotein A1)This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human hnRNP A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (92%), Pig (100%), Chicken (97%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNRPA1 antibody MGC102835 antibody hnRNP A1 antibody hnRNP-A1 antibody HNRNPA1 antibody hnRNP core protein A1-like 3 antibody hnRNP A1-like 3 antibody single-strand RNA-binding protein antibody heterogeneous nuclear ribonucleoprotein A1 antibody P |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1 antibody at 1:200 dilution.
Application
Sample(30 µg of whole cell lysate) A:H1299 B:HeLa S3 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
hnRNP A1 antibody detects HNRNPA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE hnRNP A1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. hnRNP A1 antibody diluted at 1:500.
Application
hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody diluted at 1:500.| Positive Control | H1299 , HeLaS3 , mouse brain , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
