Application
Western blot analysis of extracts of various cell lines, using HNRNPK antibody.Application
Immunohistochemistry of paraffin-embedded rat lung using HNRNPK antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human esophageal using HNRNPK antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPK antibody at dilution of 1:100 (x400 lens).Application
Immunofluorescence analysis of MCF7 cell using HNRNPK antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of A549 cell using HNRNPK antibody.| Product Name | hnRNP K antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hnRNP KThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human HNRNPK |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPRIPWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50, RIP 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUNP heterogeneous nuclear ribonucleoprotein K HNRNPK HNRPK CSBP |
|---|---|
| Target | hnRNP K |
| Molecular Weight(MW) | 51 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using HNRNPK antibody.
Application
Immunohistochemistry of paraffin-embedded rat lung using HNRNPK antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human esophageal using HNRNPK antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPK antibody at dilution of 1:100 (x400 lens).
Application
Immunofluorescence analysis of MCF7 cell using HNRNPK antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of A549 cell using HNRNPK antibody.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50, RIP 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
