Application
C. Fluorescent immunostaining of HeLa cells using anti-hnRNP RabMAb. (cat. #).Application
B. Immunohistochemical staining of paraffin-embedded human breast cancer tissue using anti-hnRNP RabMAb (cat. #).Application
A. Western blot analysis on Jurkat cell lysate using anti-hn RNP RabMAb (cat. # ) dilution 1:100000.| Product Name | hnRNP K antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP943Y] to hnRNP KThe hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants . hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals . hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway . |
| Immunogen | A synthetic peptide corresponding to residues near the N-terminus of human hnRNP was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP943Y |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| For WB: Use at a dilution of 1:100,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CSBP TUNP FLJ41122 HNRNPK HNRPK |
|---|---|
| Target | hnRNP K |
| Molecular Weight(MW) | 65 (note) |
| Tissue Specificity | This antibody detects both phosphorylated and unphosphorylated hnRNP K. |
Application
C. Fluorescent immunostaining of HeLa cells using anti-hnRNP RabMAb. (cat. #).
Application
B. Immunohistochemical staining of paraffin-embedded human breast cancer tissue using anti-hnRNP RabMAb (cat. #).
Application
A. Western blot analysis on Jurkat cell lysate using anti-hn RNP RabMAb (cat. # ) dilution 1:100000.| Application Notes | For WB: Use at a dilution of 1:100,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
