Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HNRPQ antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of HNRPQ Antibody (C-term) in Hela and Jurkat cell line lysates (35ug/lane). HNRPQ (arrow) was detected using the purified Pab.| Product Name | hnRNP Q antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to hnRNP Q (C-term)Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. |
| Immunogen | KLH conjugated synthetic peptide between 594~623 amino acids from the C-terminal region of human hnRNP Q. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | GRYRBP NS1associated protein1 hnRNPQ hnRNP Q Hetero SYNCRIP Glycine and tyrosinerich RNAbinding protein NS1-associated protein 1 Synaptotagmin-binding cytoplasmic RNA-interacting protein Glycine- and tyrosine-rich RNA-binding protein Synaptotagm |
|---|---|
| Target | hnRNP Q |
| Molecular Weight(MW) | 69.603 kDa (note) |
| Cellular Localization | The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Expressed predominantly in the nucleoplasm. Expressed predominantly in the nucleoplasm. Expressed p |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with HNRPQ antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of HNRPQ Antibody (C-term) in Hela and Jurkat cell line lysates (35ug/lane). HNRPQ (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
