Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HRH1 antibody detects HRH1 protein at cytoplasm on human hepatocellular carcinomas by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinomas. HRH1 antibody diluted at 1:500.Application
HRH1 antibody detects HRH1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HRH1 antibody diluted at 1:500.| Product Name | HRH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HRH1 (histamine receptor H1)Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HRH1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (98%), Bovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H1-R antibody hisH1 antibody HRH1 antibody "histamine receptor subclass H1 antibody" histamine H1 receptor antibody H1R antibody histamine H(1) receptor antibody HH1R antibody histamine receptor H1 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (4) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HRH1 antibody detects HRH1 protein at cytoplasm on human hepatocellular carcinomas by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinomas. HRH1 antibody diluted at 1:500.
Application
HRH1 antibody detects HRH1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HRH1 antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
