Application
Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A); MCF7 (B) whole cell lysates.Application
Immunohistochemical analysis of HSP27 (pS15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | HSP27 (phospho Ser15) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP27 (phospho Ser15)The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HSP27. The exact sequence is proprietary. |
| Modification | p-Ser15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEL-S-102 HS.76067 heat shock 27kDa protein 1 CMT2F HSPB1 SRP27 HSP27 Hsp25 HSP28 HMN2B |
|---|---|
| Target | HSP27 (phospho Ser15) |
| Tissue Specificity | Recognizes endogenous levels of HSP27 (pS15) protein. |
Application
Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A); MCF7 (B) whole cell lysates.
Application
Immunohistochemical analysis of HSP27 (pS15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Form | Liquid |
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| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
