Application
IHC-P analysis of human breast carcinoma tissue using HSP27 (phospho Ser15) antibody.Application
IHC-P analysis of human breast carcinoma tissue using HSP27 (phospho Ser15) antibody preincubated with blocking peptide.Application
WB analysis of extract from HeLa cells using HSP27 antibody (Lane 1 and 2) and HSP27 (phospho Ser15) antibody (Lane 3 and 4).Application
ICC/IF analysis of methanol-fixed HeLa cells using HSP27 (phospho Ser15) antibody.| Product Name | HSP27 (phospho Ser15) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HSP27 (phospho Ser15)The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human HSP27 around the phosphorylation site of serine15 (G -P-SP-W-D). |
| Modification | p-Ser15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Hsp25 HSP27 heat shock 27kDa protein 1 HSPB1 HSP28 HS.76067 CMT2F HMN2B SRP27 |
|---|---|
| Target | HSP27 (phospho Ser15) |
| Tissue Specificity | HSP27 (phospho-Ser15) antibody detects endogenous levels of HSP27only when phosphorylated at serine 15. |
Application
IHC-P analysis of human breast carcinoma tissue using HSP27 (phospho Ser15) antibody.
Application
IHC-P analysis of human breast carcinoma tissue using HSP27 (phospho Ser15) antibody preincubated with blocking peptide.
Application
WB analysis of extract from HeLa cells using HSP27 antibody (Lane 1 and 2) and HSP27 (phospho Ser15) antibody (Lane 3 and 4).
Application
ICC/IF analysis of methanol-fixed HeLa cells using HSP27 (phospho Ser15) antibody.| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
