Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) Antibody .Application
Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27(Phospho-Ser78) Antibody| Product Name | HSP27 (phospho Ser78) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP27 (Phospho Ser78)Involved in stress resistance and actin organization. |
| Immunogen | Peptide sequence around phosphorylation site of serine 78 (A-L-S(p)-R-Q) derived from Human HSP27. |
| Modification | p-Ser78 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMT2F DKFZp586P1322 HMN2B HS.76067 HSP27 HSP28 Hsp25 SRP27 HSPB1 28 kDa heat shock protein stress-responsive protein 27 heat shock protein beta-1 heat shock 27kD protein 1 HSP 27 heat shock 27 kDa protein estrogen-regulated 24 kDa protein h |
|---|---|
| Target | HSP27 (Phospho Ser78) |
| Molecular Weight(MW) | 27 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78) Antibody .
Application
Western blot analysis of extracts from HL60 cells untreated or treated with UV using HSP27(Phospho-Ser78) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
