Application
HSP27 [pS82] phosphospecific antibody. Peptide competition shows that only the peptide corresponding to HSP27 [pS82] blocks antibody signal, thereby demonstrating the specificity of the antibody| Product Name | Hsp27 (phospho Ser82) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Hsp27 (phospho S82)Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex. |
| Immunogen | Synthetic phospho peptide (Human). |
| Modification | p-Ser82 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMT2F DKFZp586P1322 HMN2B HS.76067 HSP27 HSP28 Hsp25 SRP27 Hsp27 (phospho S82) Hsp27 (phospho Ser82) Hsp27 (pS82) Hsp27 (pSer82) Hsp27 phospho S82 Hsp27 phospho Ser82 Phospho Hsp27 (S82) Phospho Hsp27 (Ser82) Phospho Hsp27 (pS82) Phospho |
|---|---|
| Target | Hsp27 |
| Tissue Specificity | When tested by Western blotting on TNF alpha stimulated HeLa lysates the antibody detects a single clean band of about 27kD. This band is only blocked by the phospho peptide immunogen and not by an equivalent non-phospho peptide or by a generic phospho peptide. This confirms that the antibody is specific for phospho S82 of Hsp27. |
Application
HSP27 [pS82] phosphospecific antibody. Peptide competition shows that only the peptide corresponding to HSP27 [pS82] blocks antibody signal, thereby demonstrating the specificity of the antibody| Positive Control | HeLa cells treated with TNF alpha |
|---|---|
| Application Notes | WB: 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
