Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells using HSP27 Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from Hela cells using HSP27(Ab-15) Antibody and the same antibody preincubated with blocking peptide.| Product Name | HSP27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP27Involved in stress resistance and actin organization. |
| Immunogen | Peptide sequence around aa.13~17 (G-P-S-W-D) derived from Human HSP27. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMT2F DKFZp586P1322 HMN2B HS.76067 HSP27 HSP28 Hsp25 SRP27 HSPB1 28 kDa heat shock protein stress-responsive protein 27 heat shock protein beta-1 heat shock 27kD protein 1 HSP 27 heat shock 27 kDa protein estrogen-regulated 24 kDa protein h |
|---|---|
| Target | HSP27 |
| Molecular Weight(MW) | 27 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells using HSP27 Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from Hela cells using HSP27(Ab-15) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
