Application
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Hsp90 alpha antibody diluted at 1:500.Application
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Hsp90 alpha antibody at 1:500 dilution.Application
Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 μg of Hsp90 alpha antibody . Western blot analysis was performed using Hsp90 alpha antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Hsp90 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hsp90 alpha (heat shock protein HSP 90-alpha)HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Hsp90 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chicken, Rhesus Monkey, Chimpanzee, BovinePig (100%), Chicken (90%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ31884 antibody HSP86 antibody HSP89A antibody HSP90A antibody HSP90N antibody HSPC1 antibody HSPCA antibody HSPCAL1 antibody HSPCAL4 antibody HSPN antibody Hsp89 antibody Hsp90 antibody LAP2 antibody HSP90AA1 antibody HSP 86 antibody "hea |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Hsp90 alpha antibody diluted at 1:500.
Application
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Hsp90 alpha antibody at 1:500 dilution.
Application
Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 μg of Hsp90 alpha antibody . Western blot analysis was performed using Hsp90 alpha antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 , *RK3E |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
