Application
HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSP90 beta antibody [N2C2], Internal dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human endometrial cancerrcinoma, using HSP90 beta antibody at 1:100 dilution.Application
HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP90 beta antibody [N2C2], Internal dilution: 1:500.Application
Sample(30 μg of whole cell lysate) A:HeLa S3 7.5% SDS PAGE diluted at 1:2000| Product Name | HSP90 beta antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP90 beta (heat shock protein 90kDa alpha (cytosolic), class B member 1)HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP90 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (85%), Xenopus laevis (88%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D6S182 antibody FLJ26984 antibody HSP90-BETA antibody HSP90B antibody HSPC2 antibody HSPCB antibody HSP90AB1 antibody heat shock protein HSP 90-beta antibody HSP 90 antibody heat shock protein beta antibody 90-kda heat shock protein beta HSP90 be |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSP90 beta antibody [N2C2], Internal dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HCT116, using HSP90 beta antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human endometrial cancerrcinoma, using HSP90 beta antibody at 1:100 dilution.
Application
HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP90 beta antibody [N2C2], Internal dilution: 1:500.
Application
Sample(30 μg of whole cell lysate) A:HeLa S3 7.5% SDS PAGE diluted at 1:2000| Positive Control | HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
