Application
Western blot analysis of extracts from Hela cells untreated (lane 1) or treated with hot shock (lane 2) using HSP90B (Phospho-Ser254) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using HSF1(Phospho-Ser307) Antibody| Product Name | HSP90B (phospho Ser254) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP90B (Phospho Ser254)Molecular chaperone. Has ATPase activity. |
| Immunogen | Peptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from Human HSP90B. |
| Modification | p-Ser254 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D6S182 FLJ26984 HSP90-BETA HSP90B HSPC2 HSPCB HSP90AB1 heat shock protein HSP 90-beta HSP 90 heat shock protein beta 90-kda heat shock protein beta HSP90 beta heat shock 90kD protein 1 beta HSP84 heat shock 90kDa protein 1 beta HSP 84 hea |
|---|---|
| Target | HSP90B (Phospho Ser254) |
| Molecular Weight(MW) | 92 (note) |
Application
Western blot analysis of extracts from Hela cells untreated (lane 1) or treated with hot shock (lane 2) using HSP90B (Phospho-Ser254) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using HSF1(Phospho-Ser307) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
