Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP90B Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela and A2780 cells using HSP90B Antibody .Application
Western blot analysis of extracts from Hela and A2780 cells using HSP90B(Ab-254) Antibody| Product Name | HSP90B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP90BMolecular chaperone. Has ATPase activity. |
| Immunogen | Peptide sequence around aa. 252~256 (V-G-S-D-E) derived from Human HSP90B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D6S182 FLJ26984 HSP90-BETA HSP90B HSPC2 HSPCB HSP90AB1 heat shock protein HSP 90-beta HSP 90 heat shock protein beta 90-kda heat shock protein beta HSP90 beta heat shock 90kD protein 1 beta HSP84 heat shock 90kDa protein 1 beta HSP 84 hea |
|---|---|
| Target | HSP90B |
| Molecular Weight(MW) | 92 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using HSP90B Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela and A2780 cells using HSP90B Antibody .
Application
Western blot analysis of extracts from Hela and A2780 cells using HSP90B(Ab-254) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
