Application
Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of HSPC142 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.| Product Name | HSPC142 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HSPC142 (Internal)Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component. |
| Immunogen | KLH conjugated synthetic peptide between 122~152 amino acids from the Center region of human HSPC142 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | HSPC142 BRCA1A complex subunit MERIT40 Mediator of RAP80 interactions and targeting subunit of40 kDa New component of the BRCA1A complex C19orf62 MERIT40 New component of the BRCA1-A complex NBA1 Mediator of RAP80 interactions and targeting subunit |
|---|---|
| Target | HSPC142 |
| Molecular Weight(MW) | 36.56 kDa (note) |
| Cellular Localization | Localizes at sites of DNA damage at double-strand breaks. |
Application
Flow cytometric analysis of MDA-MB468 cells using HSPC142 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain reacted with HSPC142 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of HSPC142 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HSPC142 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
