Application
Immunohistochemistry of human tonsil using anti-gamma heavy chain antibody RM116Application
ELISA of human immunoglobulins shows RM116 reacted to the γ1, γ2, γ3, γ4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
Sandwich ELISA using RM116 as the capture antibody (100ng/well), and Biotinylated antihuman light chains ( κ+ λ) antibody RM129 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.| Product Name | Human Gamma Heavy Chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM116] to Human Gamma Heavy Chain |
| Immunogen | Human IgG |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM116 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) |
|---|---|
| Target | Human Gamma Heavy Chain |
| Tissue Specificity | This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG |
Application
Immunohistochemistry of human tonsil using anti-gamma heavy chain antibody RM116
Application
ELISA of human immunoglobulins shows RM116 reacted to the γ1, γ2, γ3, γ4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
Sandwich ELISA using RM116 as the capture antibody (100ng/well), and Biotinylated antihuman light chains ( κ+ λ) antibody RM129 as the detection antibody (1ug/mL), followed by an alkaline phosphatase conjugated streptavidin.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
