Application
Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.Application
ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Product Name | Human IgG1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM117] to Human IgG1 |
| Immunogen | Peptide corresponding to the hinge region of Human IgG1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM117 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | immunoglobulin heavy constant gamma 1 (G1m marker) IGHG1 |
|---|---|
| Target | Human IgG1 |
| Tissue Specificity | This antibody reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
Application
Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
Application
ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
A titer ELISA using RM117. The plate was coated with different amounts of human IgG1. A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
