Application
Western blot analysis of MEN expression in HeLa (A); mouse testis (B) whole cell lysates.Application
Immunohistochemical analysis of MEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | 1110051B16RIK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | cDNA library was prepared and sequenced in Mouse Genome Encyclopedia Project of Genome Exploration Research Group in Riken Genomic Sciences Center and Genome Science laboratory in RIKEN.1) Katayama, S., et al., (2005) Science 309 (5740), 1564-1566. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse 1110051B16RIK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| 1110051B16RIK antibody can be used for detection of 1110051B16RIK by ELISA at 1:1562500. 1110051B16RIK antibody can be used for detection of 1110051B16RIK by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Duxbl1 |
|---|---|
| Molecular Weight(MW) | 39 kDa |
Application
Western blot analysis of MEN expression in HeLa (A); mouse testis (B) whole cell lysates.
Application
Immunohistochemical analysis of MEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Positive Control | 1) SP2/0 Cell Lysate |
|---|---|
| Application Notes | 1110051B16RIK antibody can be used for detection of 1110051B16RIK by ELISA at 1:1562500. 1110051B16RIK antibody can be used for detection of 1110051B16RIK by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store 1110051B16RIK antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
