Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using 14-3-3 zeta (Phospho-Ser58).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58).| Product Name | 14 3 3 zeta (phospho Ser58) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.1) Gu YM, et al. (2006) FEBS Lett ; 580 (1): 305-310Powell DW, et al. (2003) Mol Cell Biol; 23 (15): 5376-5387Mackintosh C. (2004) Biochem J; 381 (Pt 2): 329-342. |
| Immunogen | 14-3-3 zeta (Phospho-Ser58) antibody was raised against a peptide sequence around phosphorylation site of serine 58 (R-S-S (p) -W-R) derived from Human 14-3-3 zeta. |
| Modification | p-Ser58 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YWHAZ |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Tissue Specificity | This antibody detects endogenous level of 14-3-3 zeta only when phosphorylated at serine 58. |
| Entrez Gene | 7534 |
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| Protein Accession | NP_001129171.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using 14-3-3 zeta (Phospho-Ser58).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
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| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
