Application
Application
Western blot analysis of extracts from Jurkat cells treated with UV using Phospho-14-3-3 zeta/ delta (Thr232) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | 14-3-3 zeta/ delta (phospho Thr232) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.1) Sluchanko NN, et al. (2011) Arch Biochem Biophys 506, 24-34 |
| Immunogen | 14-3-3 zeta/ delta (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine232 (S-D-T (p)-Q-G) derived from Human 14-3-3 zeta/ delta . |
| Modification | p-Thr232 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YWHAZ |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Tissue Specificity | The antibody detects endogenous level of 14-3-3 zeta/ delta only when phosphorylated at threonine 232. |
| Entrez Gene | 7534 |
|---|---|
| Protein Accession | NP_001129171.1 |
Application
Application
Western blot analysis of extracts from Jurkat cells treated with UV using Phospho-14-3-3 zeta/ delta (Thr232) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
