Application
Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 μg/mL.Application
Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.Application
Immunofluorescence of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 μg/ml.| Product Name | 4E-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 4E-BP1 Antibody: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.1) Shah OJ, Anthony JC, Kimball SR, et al. 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am. J. Physiol. Endocrinol. Metab. 2000; 279:E715-29. |
| Immunogen | 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. The immunogen is located within the last 50 amino acids of 4E-BP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4EBP1 |
|---|---|
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 μg/mL.
Application
Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
Application
Immunofluorescence of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | 4E-BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | 4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
