Application
WB analysis of endogenous Htt lysates with or without different caspase activity (lanes 1-5) and overexpressed recombinant Htt fragment lysates (lanes 6-9) (20 ug per well) using pan-Huntingtin antibody (top, N-terminal HTT) or Huntingtin (neoepitope 552) antibody at a dilution of 1:500 (bottom, neoHtt552).Application
IHC analysis of Cortex tissue from transgenic HD mice of the YAC128 line using Huntingtin (neoepitope 552) antibody at a dilution of 1:50 (green). Tissues were counterstained with nuclei stain (blue).Application
ICC/IF analysis of Caspase cleaved Htt (green) in 293T cells transfected with an Htt23Q (left) and Htt148Q (right) using Huntingtin (neoepitope 552) antibody at a dilution of 1:50 (green). Nuclei (blue) were stained with Hoechst dye.Application
Sandwich ELISA was performed with a Huntingtin monoclonal antibody and Huntingtin (neoepitope 552) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo552 in 293T cells overexpressing the Htt construct.| Product Name | Huntingtin (neoepitope 552) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Huntingtin (neoepitope 552)Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may no |
| Immunogen | Synthetic peptide conjugated to KLH via cysteine corresponding to residues SDPAMDLND (544-552) of Human HTT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | huntingtin HD HTT IT15 |
|---|---|
| Target | Huntingtin (neoepitope 552) |
Application
WB analysis of endogenous Htt lysates with or without different caspase activity (lanes 1-5) and overexpressed recombinant Htt fragment lysates (lanes 6-9) (20 ug per well) using pan-Huntingtin antibody (top, N-terminal HTT) or Huntingtin (neoepitope 552) antibody at a dilution of 1:500 (bottom, neoHtt552).
Application
IHC analysis of Cortex tissue from transgenic HD mice of the YAC128 line using Huntingtin (neoepitope 552) antibody at a dilution of 1:50 (green). Tissues were counterstained with nuclei stain (blue).
Application
ICC/IF analysis of Caspase cleaved Htt (green) in 293T cells transfected with an Htt23Q (left) and Htt148Q (right) using Huntingtin (neoepitope 552) antibody at a dilution of 1:50 (green). Nuclei (blue) were stained with Hoechst dye.
Application
Sandwich ELISA was performed with a Huntingtin monoclonal antibody and Huntingtin (neoepitope 552) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo552 in 293T cells overexpressing the Htt construct.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C |
| Storage Buffer | PBS with 1mg/ml BSA |
