Application
Sample (30 ug of whole cell lysate) A: 293T B: Jurkat 10% SDS PAGE diluted at 1:1000| Product Name | ICAD antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ICAD (DNA fragmentation factor, 45kDa, alpha polypeptide)Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ICAD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (94%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DFF-45 antibody DFF1 antibody ICAD antibody DFFA antibody DNA fragmentation factor 45 kDa subunit antibody inhibitor of CAD antibody DNA fragmentation factor subunit alpha antibody DFF45 antibody "DNA fragmentation factor 45kDa alpha polypeptide |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: 293T B: Jurkat 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
