Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.Application
IDH1 antibody detects IDH1 protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. IDH1 antibody diluted at 1:500.Application
IDH1 antibody detects IDH1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. IDH1 antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IDH1 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: JC B: C2C12 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IDH1 antibody detects IDH1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
IDH1 antibody detects IDH1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | IDH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IDH1 (isocitrate dehydrogenase 1 (NADP+), soluble)Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Sheep, Rhesus Monkey, BovineRat (97%), Zebrafish (85%), Xenopus laevis (88%), Sheep (95%), Rhesus Monkey (100%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IDCD antibody, IDH antibody, IDP antibody, IDPC antibody, PICD antibody, IDH1 antibody, isocitrate dehydrogenase [NADP] cytoplasmic antibody, "NADP-dependent isocitrate dehydrogenase, peroxisomal antibody", NADP(+)-specific ICDH antibody, "NADP-dependent i |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Peroxisome |
Application
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-mouse IgG (HRP) was used as a secondary reagent.
Application
IDH1 antibody detects IDH1 protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. IDH1 antibody diluted at 1:500.
Application
IDH1 antibody detects IDH1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. IDH1 antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IDH1 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: JC B: C2C12 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IDH1 antibody detects IDH1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
IDH1 antibody detects IDH1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract 10% SDS-PAGE IDH1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
