Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using I kappa B- alpha (Phospho-Ser32/Ser36) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela cells untreated or treated with TNF using IkB-alpha (Phospho-Ser32/Ser36) Antibody.Application
Immunofluorescence staining of methanol-fixed Hela cells using I kappa B- alpha (Phospho-Ser32/Ser36) Antibody .| Product Name | IKB alpha (phospho Ser32/Ser36) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKB alpha (phospho Ser32/Ser36)Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. |
| Immunogen | Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human I kappa B alpha . |
| Modification | p-Ser32/Ser36 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKBA MAD-3 NFKBI NFKBIA NF-kappa-B inhibitor alpha IkappaBalpha I-kappa-B-alpha nuclear factor of kappa light chain gene enhancer in B-cells ikB-alpha major histocompatibility complex enhancer-binding protein MAD3 nuclear factor of kappa light po |
|---|---|
| Target | I kappa B alpha (Phospho Ser32/Ser36) |
| Molecular Weight(MW) | 39 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using I kappa B- alpha (Phospho-Ser32/Ser36) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela cells untreated or treated with TNF using IkB-alpha (Phospho-Ser32/Ser36) Antibody.
Application
Immunofluorescence staining of methanol-fixed Hela cells using I kappa B- alpha (Phospho-Ser32/Ser36) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
