Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK alpha (Phospho-Thr23) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK alpha (Phospho-Thr23) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23)| Product Name | IKK alpha (Phospho Thr23) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK alpha (Phospho Thr23)Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 23 (L-G-T(p)-G-G) derived from Human IKK alpha . |
| Modification | p-Thr23 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IKBKA IKK-alpha IKK1 IKKA NFKBIKA TCF16 CHUK TCF-16 inhibitor of nuclear factor kappa-B kinase subunit alpha Nuclear factor NFkappaB inhibitor kinase alpha transcription factor 16 IKK-a kinase IkB kinase alpha subunit I-kappa-B kinase 1 I-ka |
|---|---|
| Target | IKK alpha (Phospho Thr23) |
| Molecular Weight(MW) | 85 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK alpha (Phospho-Thr23) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK alpha (Phospho-Thr23) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated or treated with TNF using IKK a(Phospho-Thr23)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
