| Product Name | IKK alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IKK alphaNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa and bacteria product LPS. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb). IKKa and IKKb interact with each other and both are essential for the NF-kB activation. IKKa specifically phosphorylates IkB-a. IKKa is expressed in a variety of human tissues. |
| Immunogen | Synthetic peptide corresponding to amino acids 699-715 of human IKK alpha (1,2), which differs from correspondingmurine sequence by one amino acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested working dilution(s): Western blotting 1/500 - 1/1000: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Gene Synonyms | IKBKA IKK-alpha IKK1 IKKA NFKBIKA TCF16 IKK alpha IKKalpha |
|---|---|
| Target | IKK alpha |
| Tissue Specificity | Recognises human IKK alpha and does not cross react with IKK beta or IKK gamma. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Whole cell lysate from HeLa cells. |
|---|---|
| Application Notes | Suggested working dilution(s): Western blotting 1/500 - 1/1000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
