Application
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IKK alpha RabMAb (cat. #).Application
Western blot analysis on (A) Jurkat (B) HeLa (C) HepG2 and (D) Daudi cell lysates using anti-IKK alpha RabMAb (cat. #).| Product Name | IKK alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR464] to IKK alphaIKK alpha (I-Kappa-B kinase alpha) is a member of the IKK complex that is composed of IKK alpha, IKK beta, IKK gamma and IKAP . Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. Activation of NFKB by TNF requires phosphorylation of IKK alpha at threonine 23 and serine 176 by AKT1 and NIK, respectively . It has been demonstrated that IKK alpha can accumulate in the nucleus after cytokine exposure . In the nucleus, IKK alpha interacts with CREB-binding protein in conjunction with RELA to be recruited to the NF-kappa-B-responsive promoters in order to mediate cytokine-induced phosphorylation and subsequent acetylation of specific residues in histone H3 . |
| Immunogen | A synthetic peptide corresponding to residues in human IKK alpha was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR464 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| For WB: Use at a dilution of 1:1,000 - 10,000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFKBIKA IKKalpha IKK1 IKKA CHUK IKBKA IKK-alpha TCF16 IKK alpha |
|---|---|
| Target | IKK alpha |
| Molecular Weight(MW) | 85 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IKK alpha RabMAb (cat. #).
Application
Western blot analysis on (A) Jurkat (B) HeLa (C) HepG2 and (D) Daudi cell lysates using anti-IKK alpha RabMAb (cat. #).| Application Notes | For WB: Use at a dilution of 1:1,000 - 10,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
