Application
Western blot analysis of IKK beta (pY188) expression in HeLa LPS-treated (A); Raw264.7 LPS-treated (B); PC12 LPS-treated (C) whole cell lysates.Application
Immunohistochemical analysis of IKK beta (pY188) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | IKK beta (phospho Tyr188) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IKK beta (phospho Tyr188)The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKK beta. The exact sequence is proprietary. |
| Modification | p-Tyr188 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IKK2 IKKB IMD15 IKK-beta inhibitor of kappa light polypeptide gene enhancer in B-cells kinase beta NFKBIKB IKBKB |
|---|---|
| Target | IKK beta (phospho Tyr188) |
| Tissue Specificity | Recognizes endogenous levels of IKK beta (pY188) protein. |
Application
Western blot analysis of IKK beta (pY188) expression in HeLa LPS-treated (A); Raw264.7 LPS-treated (B); PC12 LPS-treated (C) whole cell lysates.
Application
Immunohistochemical analysis of IKK beta (pY188) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
